Sodium Bicarbonate Solution (7.5%)

Sodium bicarbonate is a widely used buffering agent in cell culture systems. It helps maintain physiological pH by neutralizing acidic by-products generated during cellular metabolism. As cells proliferate, metabolic CO2 accumulates in the culture environment, dissolves into the medium, and forms carbonic acid, which can lower pH and adversely affect cell viability and growth characteristics. Supplementation with sodium bicarbonate counteracts these fluctuations, particularly under controlled CO2 incubation conditions.

In addition to its buffering capacity, sodium bicarbonate serves as a source of carbonate ions, which are essential for various biosynthetic pathways in mammalian cells. This makes it an important component in many basal and complex cell culture media formulations.

This solution is sterile-filtered and ready for direct use in cell culture media and general laboratory applications.