Ribonucleoside Vanadyl Complexes

Ribonucleoside Vanadyl Complex (RVC) is a commonly used ribonuclease (RNase) inhibitor. It is widely applied in the isolation, purification and detection of RNA samples to inhibit RNA degradation. This product is supplied at a concentration of 200 mM, and is prepared by optimally reacting an equimolar mixture of four rNTPs with vanadium oxide. It binds to RNase via non-covalent bonds to form stable complexes, thereby suppressing the activity of most RNases. In addition, this product does not inhibit DNase I. Therefore, when DNase I is used to digest contaminating DNA in RNA samples, RVC can be added to block RNase activity and protect RNA from degradation.