RI-1
RI-1 (CAS: 415713-60-9) is a small molecule inhibitor targeting RAD51, a protein essential for homologous recombination and DNA repair in eukaryotic cells. RI-1 acts by forming a covalent bond with cysteine 319 on the surface of RAD51, disrupting the protein-protein interfaces required for filament formation and recombinase activity. In vitro, RI-1 inhibits RAD51 function with IC50 values ranging from 5 to 30 μM, impeding the assembly of RAD51 foci following DNA damage and sensitizing tumor cells to DNA crosslinking agents. Due to its activity in various human cancer cell lines, RI-1 is a valuable tool for studying RAD51-mediated DNA repair pathways in preclinical research.
Reference:
[1] Budke B, Logan HL, Kalin JH, Zelivianskaia AS, Cameron McGuire W, Miller LL, Stark JM, Kozikowski AP, Bishop DK, Connell PP. RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res. 2012;40(15):7347-57.
[2] Christian Jekimovs, Emma Bolderson, Amila Suraweera, Mark Adams, Kenneth J. O’Byrne, and Derek J. Richard. Chemotherapeutic Compounds Targeting the DNA Double-Strand Break Repair Pathways: The Good, the Bad, and the Promising. Front Oncol. 2014; 4: 86.
| Storage | Store at -20°C |
| M.Wt | 361.61 |
| Cas No. | 415713-60-9 |
| Formula | C14H11Cl3N2O3 |
| Solubility | insoluble in H2O; ≥18.1 mg/mL in DMSO; ≥8.89 mg/mL in EtOH with gentle warming |
| Chemical Name | 3-chloro-1-(3,4-dichlorophenyl)-4-morpholin-4-ylpyrrole-2,5-dione |
| SDF | Download SDF |
| Canonical SMILES | O=C(C(N1CCOCC1)=C(C1=O)Cl)N1c(cc1)cc(Cl)c1Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Description | RI-1 is a cell-permeable inhibitor of RAD51. | |||||
| Targets | RAD51 | |||||
| IC50 | 5~30 μM | |||||
Quality Control & MSDS
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Chemical structure
