Recombinant Rhesus Macaque gamma-Interferon Inducible Protein 10/CXCL10
CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The rhesus macaque IP-10 shares 69 %, 95 %, 74 % a.a. sequence identity with human, murine, rat IP-10.
Reference
1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6
2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71
3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25
4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204
5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62
6. Dinarello CA. 1994. FASEB J. 8:1314-25.
| Gene ID | 574243 |
| Accession # | Q8MIZ1 |
| Alternate Names | Gamma-IP10, Small-inducible Cytokine B10 |
| Source | Escherichia coli. |
| M.Wt | Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids. |
| AA Sequence | IPLSRTVRCT CISISNQPVN PRSLEKLEII PPSQFCPHVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRhIP-10/CXCL10 as determined by LAL method.