Recombinant Rat Thymus and Activation Regulated Chemokine/CCL17
CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309.
Reference
1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308
2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21
3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21
4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8
5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.
| Gene ID | 117518 |
| Accession # | Q9ERE0 |
| Source | Escherichia coli. |
| M.Wt | Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| AA Sequence | ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/μg of r rRtTARC/CCL17 as determined by LAL method.