Recombinant Rat SDF-1α/CXCL12α
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.
Reference:
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.
2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.
3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.
4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.
5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.
6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.
7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.
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Gene ID |
24772 |
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Accession # |
Q9QZD1 |
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Alternate Names |
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Source |
Escherichia coli. |
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M.Wt |
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
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AA Sequence |
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.