Recombinant Rat Macrophage-Derived Chemokine/CCL22
CCL22 is a protein encoded by the CCL22 gene. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).
Reference
1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1
2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604
3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14
4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.
| Alternate Names | STCP-1, MDC (1-69), Macrophage-derived Chemokine |
| Source | Escherichia coli. |
| M.Wt | Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids. |
| AA Sequence | GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRtMDC/CCL22 as determined by LAL method.