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Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1

Catalog No.
P1513
 
Grouped product items
SizePriceStock Qty
5ug
$99.00
Ship with 5-10 days
100ug
$813.00
Ship with 5-10 days
500ug
$1,825.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Rat CXCL1, also known as CINC-1, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. It was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells. CINC-1 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of rat CXCL1 is approximately 68 % identical to the human CXCL1.

Reference

1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6

2. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98

3. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9

4. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83

5. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802

6. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44.

Information

Gene ID81503
Accession #P14095
Alternate NamesGRO-alpha, Platelet-derived Growth Factor-inducible Protein KC
SourceEscherichia coli.
M.WtApproximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.
AA SequenceAPVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK
AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
FormulationLyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
Shipping ConditionGel pack.
HandlingCentrifuge the vial prior to opening.
UsageFor Research Use Only! Not to be used in humans.

Quality Control

Quality Control & DataSheet

View current batch:
    Purity > 97 % by SDS-PAGE and HPLC analyses.
    Endotoxin: Less than 1 EU/μg of rRtCINC-1/CXCL1 as determined by LAL method.
  • Datasheet