Recombinant Mouse IL-5 (His, Strep)

Interleukin 5 (IL-5) is a secreted glycoprotein that belongs to the α-helix class of cytokines. Unlike other family members, it exists in the form of covalently attached antiparallelismamers. The mouse IL-5 gene encodes a signal peptide and a 113-amino acid (AA) mature protein. Mature mouse IL-5 exhibits amino acid sequence homology of 70%, 94%, 58%, 66%, 59%, and 63% to human, rat, canine, horse, feline, and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptors. IL-5 is predominantly produced by CD4+ Th2 cells, but can also be produced by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin's disease, and IL-2-stimulated invariant natural killer T cells (INKTs). IL-5 increases the production and mobilization of eosinophils and CD34+ progenitor cells in the bone marrow and promotes the maturation of eosinophil precursors outside the bone marrow. The human IL-5 receptor is predominantly expressed by eosinophils and is also present on basophils and mast cells, consisting of a unique ligand-binding subunit (IL-5Rα) and a common signal transduction subunit βc. IL-5Rα first binds to IL-5 with low affinity and then binds to a pre-formed β-dimer to form a high-affinity receptor.