Recombinant Human MIP-4/CCL18
Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immunity responses.
Reference
[1]. Hieshima K, Imai T, Baba M, et al. 1997. J Immunol, 159: 1140-9.
[2]. Chenivesse C, Chang Y, Azzaoui I, et al. 2012. J Immunol, 189: 128-37.
[3]. Azzaoui I, Yahia SA, Chang Y, et al. 2011. Blood, 118: 3549-58.
[4]. Adema GJ, Hartgers F, Verstraten R, et al. 1997. Nature, 387: 713-7.
| Accession # | P1219 |
| Alternate Names | AMAC-1, PARC, DC-CK1 |
| Source | Escherichia coli. |
| Protein sequence | AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA |
| M.Wt | 7.9 kDa |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Avoid repeated freeze-thaw cycles It is recommended that hat the protein be aliquoted for optimal storage - 12 months from date of receipt, -20 to -7°C as supplied |
| Concentration | 0.1-1.0 mg/mL |
| Formulation | Dissolved in Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml. |
| Shipping Condition | Blue ice |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <1 EU per 1 ug of the protein by the LAL method.