Recombinant Human Liver-Expressed Chemokine/CCL16
Human CCL16, also called Liver-expressed chemokine (LEC), Monotactin-1 (MTN-1), IL-10-inducible chemokine and so on, is expressed by the CCL16 gene located on the chromosome 17 in humans. The gene encodes a 120 a.a. residue precursor protein with a 23 a.a. residue predicted signal peptide that is cleaved to generate a 97 a.a. residue mature protein. The protein is secreted by the liver, thymus, spleen cells and showing chemotactic activity for lymphocytes and monocytes but it is distantly related to other CC chemokines, exhibiting less than 30 % sequence identity. CCL16 is highly induced by IL-10, IFN-γ and bacterial lipopolysaccharide in monmcytes and signal through CCR1, CCR2, CCR5, and CCR8.
Reference
1. Hedrick JA, Helms A, Vicari A, et al. 1998. Blood, 91: 4242-7
2. Nomiyama H, Fukuda S, Iio M, et al. 1999. J Interferon Cytokine Res, 19: 227-34
3. Youn BS, Zhang S, Broxmeyer HE, et al. 1998. Biochem Biophys Res Commun, 247: 217-22
4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579: 6044-8
5. Francica G, Petrolati A, Di Stasio E, et al. 2012. Acta Radiol, 53: 394-400.
| Gene ID | 6360 |
| Accession # | O15467 |
| Alternate Names | HCC-4, LCC-1, LMC, MTN-1, NCC-4 |
| Source | Escherichia coli. |
| M.Wt | Approximately 11.2 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids. |
| AA Sequence | QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuLEC/CCL16 as determined by LAL method.