Recombinant Human Glia Maturation Factor beta
Catalog No.
P1305
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The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.
Reference
1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7
2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8
3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25
4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82.
| Gene ID | 2764 |
| Accession # | P60983 |
| Alternate Names | GMFB |
| Source | Escherichia coli. |
| M.Wt | Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| AA Sequence | SESLVVCDVA EDLVEKLRKF RFRKETNNAA IIMKIDKDKR LVVLDEELEG ISPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Data Not Available. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuGMF-β as determined by LAL method.