Recombinant Chicken IL-21 (N-His)
Chicken interleukin-21 (Gallus gallus Interleukin-21, Chicken IL-21) is a secreted cytokine with immunomodulatory activity, belonging to the IL-2 family (IL-15/IL-21 family) of four-helix bundle cytokines. Functionally, the main activities of chicken IL-21 come from experimental studies: Rothwell et al. (2012, Dev Comp Immunol) demonstrated that chicken IL-21 has a co-stimulatory effect on T cells, synergistically enhancing T cell proliferation and blocking dendritic cell maturation; Shi and Erf (2012, J Invest Dermatol) found in the Smyth line chicken spontaneous vitiligo model that IL-21 is co-expressed and elevated with IFN-γ and IL-10 in the early and active phases of lesions, suggesting that vitiligo is a Th1-polarized autoimmune disease and that IL-21 participates in its early active phase. In terms of application, chicken IL-21 has potential value as an avian immune adjuvant and vaccine enhancer, and its property of suppressing DC maturation warrants further exploration in avian immune tolerance and autoimmune research.
| Gene ID | 001024835.2 |
| Accession # | NP_001020006.2 |
| Alternate Names | Chicken IL-21, IL21, Interleukin-21 |
| Source | E.coli |
| Protein sequence | MERMIIFCML FFCFSMVLTA TSPKAMKYKQ LSKTIDHLKD VVKDKDVELL HTPENPGDGC LLTAVTCFQN GILKLQPRNS QVNATFAKTV KILRRPFLPV SEEHCESSCE SYERKKPQEF LNSFSKLMQK LFKNSTAERY GKSTI |
| M.Wt | Approximately 18.9 kDa |
| Appearance | Solution protein |
| Stability & Storage | Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. -3 years from date of receipt, -20 to -70°C as supplied. |
| Concentration | 5 mg/mL |
| Formulation | Supplied as a 0.2 μm filtered solution in PBS, pH7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
| Biological Activity | Significantly upregulates IL-2, IL-4, IL-10, IL-17A, IFN-γ, and TGF-β1 mRNA levels; significantly upregulates IL-1β, IL-6, and TNF-α mRNA levels. |
| Shipping Condition | Shipping with dry ice. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
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