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Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

Catalog No.
K2223
Detects pyruvate kinase activity, sensitive, high throughput screening (HTS).
Grouped product items
SizePriceStock Qty
100 assays
$1,658.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Pyruvate kinase (PK) is an important enzyme involved in glycolysis metabolic pathway. PK catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, producing ATP and pyruvate. Lack of pyruvate kinase will cause the disease pyruvate kinase deficiency which slows down the process of glycolysis.

 The Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit provides a sensitive, simple, fast and convenient way for accurate detection of PK activity in various samples (blood, tissues, and culture cells, etc.) based on colorimetric and fluorometric method. In the assay, PEP and ADP were catalyzed by PK to produce ATP and pyruvate, which is subsequently oxidized by pyruvate oxidase to generate fluorescence (Ex/Em = 535/587 nm) and color (λ = 570 nm). The increase in fluorescence intensity or color is proportional to the increase in pyruvate amount. The kit can detect 0.1 mU/mL pyruvate kinase.

Features & Properties

FeaturesFast and convenient; Simple procedure; Takes approx. less than 40 minutes.
ShippingBlue Ice
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Storage

Store at -20°C.

Overview

Kit componentsPK Assay Buffer, OxiRed™ Probe (in DMSO), PK Enzyme Mix, PK Substrate Mix, PK Positive Control, Pyruvate Standard (100 nmol/µL)