Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Pyruvate dehydrogenase (PDH) is a central enzyme in mitochondrial energy metabolism, catalyzing the oxidative decarboxylation of pyruvate to acetyl-CoA. This reaction bridges glycolysis and the tricarboxylic acid (TCA) cycle, playing a critical role in cellular respiration. Dysregulated PDH activity is associated with various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Accurate detection of PDH activity is essential for studying cellular energy metabolism, disease diagnosis, and drug development.

This kit can measure PDH activity in serum, plasma, tissue, and cell samples by quantifying the rate of NADH production. In the PDH-catalyzed reaction, pyruvate is decarboxylated, and NAD⁺ is reduced to NADH. The generated NADH then reacts with a chromogenic substrate, producing a colored product with a characteristic absorbance at 450 nm. By monitoring the increase in absorbance at 450 nm over time, PDH activity can be calculated based on the rate of NADH generation. 

Figure 1. Typical standard curve and assay data. (A) The reference standard curve, (B) The PDH activity in different samples generated using this kit.