Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit

Pyruvate Dehydrogenase (PDH) plays an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH forms a well-characterized enzyme complex. In the presence of NAD⁺ and CoA, PDH converts pyruvate into acetyl-CoA. PDH also links glycolysis metabolic pathway to the citric acid cycle. PDH activity is inhibited by high ratios of intracellular Acetyl-CoA/CoA, NADH/NAD⁺ or ATP/ADP. In humans, PDH deficiency inhibits mitochondrial function and is involved in neurodegenerative diseases. PDH deficiency is X-linked and results in 2 forms of abnormality: seizure and/or neuropathological spasm (a neurological form) and lactic acidosis (a metabolic form). PDH is also a target of oncogene-induced senescence. Activation of PDH can increase respiration and redox stress, and enhance pyruvate utilization.

 The Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection PDH activity in various samples based on colorimetric method. In the assay, PDH changes pyruvate to an intermediate which reduces the developer to a colored (450 nm) product. The kit can detect PDH activity lower than 0.1 mU in a variety of samples.