Toggle Nav
Close
  • Menu
  • Setting

Pyruvate Colorimetric/Fluorometric Assay Kit

Catalog No.
K2119
Detects pyruvate, high throughput screening (HTS).
Grouped product items
SizePriceStock Qty
100 assays
$1,447.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Pyruvate is an important intermediate in several metabolic pathways. Pyruvate is produced from glucose via glycolysis. Pyruvate can be converted to fatty acids via acetyl CoA or to carbohydrates via gluconeogenesis. Pyruvate is also used to stimulate metabolism which then leads to loss of body weight. High levels of pyruvate are associated with genetic disorders and liver disease.

The Pyruvate Colorimetric/Fluorometric Assay Kit provides a simple and convenient way for detection of pyruvate in various biological samples such as culture and fermentation media, blood and cells based on colorimetric and fluorometric method. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to yield fluorescence (at Ex/Em = 535/587 nm) and color (at λ = 570 nm). The fluorescence or color intensity is proportional to pyruvate content. The assay provides a direct and automation-ready procedure for accurately measuring pyruvate concentration. The kit can detect 1 µM to 10 mM pyruvate.

Features & Properties

FeaturesFast and convenient; Simple procedure; Takes approx. 40 minutes; Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples.
ShippingBlue Ice
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Storage

Store at -20°C.

Overview

Kit componentsPyruvate Assay Buffer, Pyruvate Probe (in DMSO), Pyruvate Enzyme Mix, Pyruvate Standard (100 nmol/µL)