Protein Carbonyl Content Assay Kit

Protein carbonyl groups are an essential and immediate biomarker for oxidative stress. DNPH tagging of proteins is a common way of measuring oxidative stress.  DNP hydrazones formed from the reaction can be easily quantified at 375 nm. The Protein Carbonyl Content Assay Kit offers an easy and accurate procedure for quantifying carbonyls in protein samples. For example, The detection limit of a 1 mg (approx. 15 nmol) of BSA sample is about 0.15 nmol carbonyl, and BSA typically contains approximately 1-3 nmol carbonyl/mg.