PNU112455A hydrochloride

PNU112455A hydrochloride (CAS No.: 21886-12-4) is an aminopyrimidine-based small molecule that functions as an ATP-competitive inhibitor of cyclin-dependent kinases CDK2 and CDK5, key regulators of cell cycle progression and neuronal signaling. By binding to the ATP-binding sites of CDK2 and CDK5, it interferes with kinase catalytic activity, exhibiting inhibitory potency in the low micromolar range and demonstrating competitive kinetics with respect to ATP. This compound has been shown to modulate phosphorylation-dependent processes, including suppression of tau hyperphosphorylation and attenuation of glucocorticoid-induced cytotoxicity in neuronal cell models, supporting its relevance in studies of neurodegeneration and cell survival pathways. PNU112455A hydrochloride is widely used as a research tool to investigate CDK-mediated signaling cascades, kinase-substrate interactions, and mechanisms of cell cycle and neuronal dysfunction, with applications in in vitro biochemical assays and cell-based models where experimental concentrations are selected according to specific assay conditions and study objectives.