PKH26 Red Fluorescent Cell Linker Kit
- 1. Pengfei Ge, Jingfan Wang, et al. "Targeted delivery of miR-199a-5p by RGD-modified HUMSC-extracellular vesicles regulates ocular neovascularization via suppression of endothelial–mesenchymal transition." Materials & Design Volume 265, May 2026, 115878
- 2. Yi Jin, Guochen Han, et al. "Serum-tolerant polymeric complex for stem-cell transfection and neural differentiation." Nat Commun. 2025 Feb 27;16(1):2022. PMID: 40016275
PKH26 Red Fluorescent Cell Linker Kit is a kit that uses the red fluorescent probe PKH26 for labeling cell membranes. PKH26 can bind to lipid regions of cell membranes and show fluoresce red. PKH26 is less toxic to cells, has a low fluorescence background, and does not stain neighboring cells, so it is widely used for cell tracing in vitro and in vivo. PKH26-labeled cells have good morphology and stable fluorescence expression, which can effectively observe the situation of cells in vitro; PKH26-labeled cells can also be used for in vivo observation for up to several weeks. Meanwhile, the fluorescence of PKH26 is evenly distributed to daughter cells with cell division, and the fluorescence signal is related to the cell cycle state. Based on this property, PKH26 can also be used to detect cell proliferation. PKH26 can also be used in combination with PKH67 for experiments such as labeling cells, detecting proliferation, and cell tracing.

Figure 1: Diagram of the kit for labeling Hela cells
| Components |
K2410-25 T |
K2410-250 T |
|
PKH26 |
100 μL |
1 mL |
|
Diluent |
50 mL |
500 mL |
|
Store the kit at -20°C, stable for 1 year. PKH26 should be stored away from light and moisture. |
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