Pipemidic acid

Pipemidic acid (CAS No.: 51940-44-4) is a synthetic small molecule antibacterial research compound in the pyridopyrimidine related quinolone class and a structural derivative of piromidic acid that is widely used as a tool molecule in microbiology and anti infective discovery workflows, with primary activity centered on inhibition of bacterial DNA gyrase, a type II topoisomerase that controls ATP dependent negative DNA supercoiling and thereby regulates replication and transcription dynamics in susceptible bacteria; consistent with this mechanism, it shows activity against Gram negative organisms including Pseudomonas aeruginosa and selected Gram positive strains, and in research settings it is commonly categorized as a DNA topology modulator for studies of bacterial growth control, resistance phenotypes, and gyrase linked stress responses rather than as a eukaryotic signaling probe, while quantitative potency is typically reported through antimicrobial susceptibility endpoints such as MIC values that are generally described across low micromolar to higher micromolar equivalent exposure ranges depending on strain background, inoculum conditions, and media composition when exact experiment specific values are not harmonized across sources; typical use cases include in vitro bacterial culture assays, comparative profiling against reference antibacterials, resistance selection and mechanism mapping studies, and occasional in vivo infection model benchmarking in preclinical pharmacology contexts, where the concentrations or doses used in experiments typically depend on specific experimental designs and research objectives.