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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
Melanoma (WM9), prostate carcinoma (DU145 and C4–2), ovarian carcinoma (Hey), colon carcinoma (WM480), and lung carcinoma (A549)
Preparation method
The solubility of this compound in DMSO is ≥13.5mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
10 μg/ml
Applications
The growth of all six of the cell lines in culture was inhibited by pentamidine in a concentration-dependent manner with complete growth inhibition of the cell lines occurring at 10 μg/ml. These results demonstrated an in vitro growth-inhibitory activity of pentamidine against different human cancer cell lines that expressed the endogenous PRL phosphatases.
Animal models
Athymic nude mouse model
Dosage form
6–10 mg/kg (i.m.), once every two days
Application
At the 16th week of the study, the tumor size of the experimental mice treated with Pentamidine was similar to that at the beginning of the treatment, while the tumors of the control mice grew so fast that humanitarian sacrifices were required at 4 weeks. At the end of the study period, there were no significant histological changes in the internal organs (heart, kidney, liver, lung, and spleen) of Pentamidine-treated mice. The tumors of these mice were evaluated and found that necrosis accounted for more than 50% of the total tumors, while the tumors of untreated control mice did not.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Pathak MK, Dhawan D, Lindner DJ, Borden EC, Farver C, Yi T. Pentamidine is an inhibitor of PRL phosphatases with anticancer activity. Mol Cancer Ther. 2002 Dec;1(14):1255-64. PubMed PMID: 12516958.