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PDGF-BB, murine recombinant protein

Catalog No.
PDGF-BB, platelet-derived growth factor, expressed in E.Coli.
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The platelet-derived growth factor BB (PDGF-BB) is a member of the PDGF family and is a disulfide-linked homodimer. PDGF family consists of proteins PDGF-A, -B that form three disulfide-linked homodimers (PDGF-AA, -BB and -AB). The PDGFs are stored in platelet α-granules, and are released upon platelet activation. PDGFs signals through receptors PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. However, PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF-BB is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells and some blood cells.

Recombinant murine PDGF-BB is a homodimeric, non-glycosylated polypeptide of 109 amino acids and having a molecular mass of 24.4 kDa.

Biological Activity

Determined by the dose-dependent proliferation of murine BALB/c 3T3 cells.
ED50 < 2 ng/ml.          

Quality Control

Quality Control & DataSheet

View current batch:
    Purity: ≥95%, by SDS-PAGE and HPLC.
    Endotoxin Level:< 0.1 ng/μg/span>

Related Biological Data



Gene ID: 18591 Accession #: P31240
Alternate Names:PDGFB, Murine PDGF-BB, m-PDGF-BB, PDGFBB, PDGF-BB, PDGF BB, rm-PDGF-BB, recombinant murine PDGF-BB, recombinant PDGF-BB, PDGF.
Source: Escherichia Coli. M.Wt: 24.4 kDa
Appearance: Lyophilized protein Storage: Store at -20°C
Formulation:Lyophilized with no additives.
Solubility:Reconstitute in sterile 100mM acetic acid and 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Shipping Condition: Gel pack. Handling: Centrifuge the vial prior to opening.
Usage:For Research Use Only! Not to be used in humans.