Neutral Buffered Formalin Fixative (10%)

The purpose of fixation is to preserve the original morphological structure of cells and tissues. Fixatives prevent autolysis of tissues and cells by endogenous lysosomal enzymes while also inhibiting the growth of bacteria and molds. Fixatives alter the internal structure of proteins through mechanisms such as coagulation and the formation of additive compounds, thereby inactivating enzymes. In addition, fixatives physically alter the nucleus and extracellular components. Commonly used fixatives include aldehydes, mercury-containing compounds, alcohols, oxidizing agents, and picrate salts. Among these, formaldehyde (an aldehyde) and ethanol (an alcohol) are the most frequently used.

Neutral Buffered Formalin (10%) is mainly composed of formaldehyde, phosphate salts, and deionized water. It is suitable for fixing most tissues.