N6-acetyllysine (H-Lys(Ac)-OH)

N6-acetyllysine (H-Lys(Ac)-OH) (CAS No.: 692-04-6) is an endogenous acetylated amino acid and a derivative of L-lysine that functions as a biologically relevant metabolite and signaling regulator in epigenetic and metabolic processes. As an R-chain N-acetylated α-amino acid, it is closely associated with protein acetylation dynamics and reflects cellular acetylation status. This molecule has been implicated in the modulation of enzyme activity, particularly through inhibition of histone deacetylases, thereby altering chromatin structure and influencing gene transcription. Through these mechanisms, N6-acetyllysine participates in the regulation of cell proliferation, differentiation, and metabolic homeostasis, making it a valuable tool in studies of cancer biology and metabolic disorders. Elevated levels of this metabolite have been observed in pathological conditions such as liver cirrhosis associated with cholestatic diseases, where its concentration correlates with disease severity, highlighting its potential as a biomarker. In vitro, its modulatory effects on enzymatic activity are typically observed in the low micromolar to millimolar range depending on the experimental system. N6-acetyllysine is widely utilized in cell-based assays and biochemical studies to investigate epigenetic regulation, protein acetylation pathways, and disease-related metabolic alterations, with applied concentrations varying according to specific experimental designs and research objectives.