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N1-Methylpseudouridine

Catalog No.
B8340
A modified nucleoside used for enhancing mRNA translation
Grouped product items
SizePriceStock Qty
10mg
$310.00
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50mg
$910.00
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Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

N1-Methylpseudouridine is a modified nucleoside used for enhancing mRNA translation. Incorporation of N1-Methylpseudouridine into mRNA turns off the immune/eIF2α phosphorylation-dependent inhibition of translation, and increases ribosome pausing and density on the mRNA, thus dramatically facilitating the translation process. N1-Methylpseudouridine outperforms several other modified nucleosides (e.g. 5-Methylcytidine) and corresponding combinations in terms of translation capacity. 

References:

1. Svitkin YV, Cheng YM, Chakraborty T, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Research, 2017, 45(10): 6023-6036.

2. Andries O, Mc Cafferty S, De Smedt SC, et al N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344.

Chemical Properties

Physical AppearanceSolution
StorageStore at -20°C or below
M.Wt258.23
Cas No.13860-38-3
FormulaC10H14N2O6
Solubility≥50 mg/mL in H2O with ultrasonic; ≥20 mg/mL in EtOH; ≥20.65 mg/mL in DMSO
Chemical Name5-((2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-1-methylpyrimidine-2,4(1H,3H)-dione
SDFDownload SDF
Canonical SMILESO=C(N1)N(C)C=C([[email protected]@H]2O[[email protected]](CO)[[email protected]@H](O)[[email protected]]2O)C1=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[2]

Cell lines

Various mammalian cells including A549, BJ, C2C12, HeLa and primary keratinocytes

Reaction Conditions

24 h incubation

Applications

In various mammalian cells, mRNAs in the N1-Methylpseudouridine + 5-Methylcytidine group showed reduced cytotoxicity compared to the Pseudouridine + 5-Methylcytidine group. The mRNAs simultaneously modified by N1-Methylpseudouridine and 5-Methylcytidine also exhibited reduced activation of the intracellular innate immune response.

Animal experiment:[2]

Animal models

7-week-old Balb/c mice

Dosage form

20 μg

Once daily through intradermal or intramuscular routes by lipofection, for 21 days

Applications

N1-Methylpseudouridine alone and its combination with 5-Methylcytidine outperformed the current state-of-the-art Pseudouridine and its combination with 5-Methylcytidine, in terms of translation capacity.

Note

The technical data provided above is for reference only.

References:

1. Svitkin YV, Cheng YM, Chakraborty T, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Research, 2017, 45(10): 6023-6036.

2. Andries O, Mc Cafferty S, De Smedt SC, et al N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344.

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