N-Phthaloyl-β-alanine

The core biological function of N-Phthaloyl-β-alanine (CAS No. 3339-73-9) is to serve as a hapten for preparing immunoconjugates (coating conjugates, enzyme conjugates), facilitating monoclonal antibody production, and subsequently used for enzyme-linked immunosorbent assay (ELISA) detection of organophosphorus pesticides (azinphos-methyl, phosmet); in animal experiments, it is used for BALB/c mouse immunization experiments (antibody preparation), with commonly used immunization doses of intraperitoneal injection 100 μg / mouse (primary immunization, emulsified with Freund’s adjuvant) and 250 μg / mouse (booster immunization, without adjuvant); as an immunoassay tool molecule, it has no effective therapeutic concentration. When preparing immunoconjugates, the initial molar ratio of hapten to ovalbumin (OVA) is 30:1, and the conjugation molar ratio to bovine serum albumin (BSA) is approximately 22-25 (immunoconjugate).

References:

[1] Mercader JV, Montoya A. Development of monoclonal ELISAs for azinphos-methyl. 1. Hapten synthesis and antibody production. J Agric Food Chem. 1999 Mar;47(3):1276-84. doi: 10.1021/jf9808675. PMID: 10552449.

[2] Bhatti MH, Yunus U, Shah SR, Fl?rke U. Caffeine-N-phthaloyl-β-alanine (1/1). Acta Crystallogr Sect E Struct Rep Online. 2012 Jun 1;68(Pt 6):o1888. doi: 10.1107/S1600536812022696. Epub 2012 May 26. PMID: 22719646; PMCID: PMC3379448.