N-Butyl-N-(4-hydroxybutyl)nitrosamine

N-Butyl-N-(4-hydroxybutyl)nitrosamine (CAS No.: 3817-11-6) is a chemical commonly used in biomedical research, primarily to induce bladder cancer in animal models. Its mechanism of action involves mutagenesis of urothelial cells, leading to high levels of mutations in bladder epithelial cells, thereby promoting tumor formation. In in vitro studies, this compound shows activity in the nanomolar to micromolar range, with specific values depending on experimental conditions and cell type. In animal models, N-Butyl-N-(4-hydroxybutyl)nitrosamine is typically administered via drinking water or intragastric gavage to induce bladder cancer. The dose or concentration used in experiments usually depends on the experimental design and research objectives. In addition, this compound is also used to study the mechanisms of bladder cancer development, screen potential therapeutic drugs, and evaluate the effectiveness of preventive measures.