Myeloperoxidase Peroxidation Fluorometric Assay Kit

Myeloperoxidase (MPO) is an important member of the heme peroxidase-cyclooxygenase superfamily, which is expressed in a variety of inflammatory cells such as neutrophils, activated microglia, monocytes, and is essential for immune surveillance and host defense. Studies have shown that MPO plays a key role in a variety of diseases and is a potential biomarker and therapeutic target for inflammatory and oxidative stress-related diseases.

MPO can catalyze two types of redox reactions, including chlorination and peroxidation. The Myeloperoxidase Peroxidation Fluorometric Assay Kit can be used to quantitatively detect MPO peroxidation activity in cell and tissue samples. The peroxidase in the sample can catalyze non-fluorescent ADHP to the fluorescent product Resorufin (excitation/emission wavelength = 535/587 nm), and the fluorescence intensity of Resorufin is proportional to the total peroxidase activity in the sample. A specific MPO inhibitor is also provided in the kit that specifically inhibits the peroxidation activity of MPO in the sample, thereby distinguishing MPO-mediated peroxidation from the activity of other peroxidases in the sample.

In addition, MPO activity can also be detected by chlorination, the Myeloperoxidase Chlorination Fluorometric Assay Kit (Cat. No. K2289).

Figure 1: MPO catalytic mechanism

Figure 2: The detection principle of this product

Figure 3: Resorufin Standard Curve

Figure 4: MPO Activity (ΔRFU +/- inhibitor) for freshly prepared Human cell (40 µL) and reagent blank