Mycoplasma LAMP Detection Kit (HNB)

Mycoplasma is the smallest prokaryotic microorganism capable of growth and reproduction in non-living culture media, with a size of only 0.1-0.3 μm. It can pass through standard sterilizing-grade filters (0.22-0.45 μm). Mycoplasma contamination alters cellular gene expression and protein function, severely impacting cell physiology. As mycoplasma is not visible to the naked eye, regular testing during cell culture is essential.

There are various methods for detecting mycoplasma contamination, such as culture, PCR, qPCR, and ELISA. However, most are time-consuming and relatively cumbersome to perform. The Mycoplasma LAMP Detection Kit (HNB) utilizes isothermal amplification technology for mycoplasma detection. In the presence of mycoplasma contamination, the conservative sequence of mycoplasma DNA will be specifically amplified by isothermal DNA polymerase, causing the reaction solution to change color from purple-blue to sky blue. The procedure is straightforward and rapid: it requires only 1 μL of culture supernatant and 1-hour incubation at 65°C to generate the result. 

Compared to commonly used nested PCR, this kit demonstrates higher tolerance to inhibitors present in the culture supernatant, minimizing the risk of false negatives. The closed-tube format eliminates the need to open the lid after amplification, greatly avoiding cross-contamination and false positives. Results can be determined by direct visual observation, making the kit highly suitable for routine mycoplasma detection in the laboratory.

Figure 1. LAMP colorimetric detection of mycoplasma