MTT
Catalog No.
B7777
reagent used in the measurement of in vitro cell proliferation
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MTT (CAS 298-93-1), chemically known as 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, is a tetrazolium salt commonly employed in biomedical research to assess cellular proliferation and metabolic activity in vitro. Upon uptake by viable cells, MTT undergoes reduction primarily mediated by NADH-dependent oxidoreductases in mitochondria, and partly by extra-mitochondrial enzymes, forming insoluble formazan precipitates. The resulting formazan accumulation correlates with metabolic activity and cell viability. Due to its membrane-permeable cationic nature, MTT efficiently penetrates intact cell membranes without requiring intermediates, distinguishing it from second-generation, negatively charged tetrazolium derivatives.
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| Storage | Store at -20°C |
| M.Wt | 414.32 |
| Cas No. | 298-93-1 |
| Formula | C18H16N5SBr |
| Solubility | ≥41.4 mg/mL in DMSO; ≥18.63 mg/mL in EtOH; ≥2.5 mg/mL in H2O with ultrasonic |
| Chemical Name | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C)N=C([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)S1.[Br-] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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