Mouse Pan B Cell Isolation Kit (Negative Selection)
The Mouse Pan B Cell Isolation Kit (Negative Selection) is used for the isolation of total B cells from Single-cell suspension of mouse spleen, lymph nodes, bone marrow cells, or peritoneal lavage fluid by negative selection. The isolation principle of this kit is to label unwanted cells with the biotin-labeled monoclonal antibody mix, and then add streptavidin-labeled magnetic beads to remove unwanted cells. Isolated cells are high-purity total B cells.
This kit is easy to use and does not require a separation column, which only requires a magnetic separator. At the same time, the obtained cells are not labeled with antibodies and magnetic beads, so they will not be abnormally activated and will not affect downstream experiments.
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Components |
50 Assays (for 5×108 cells) |
100 Assays (for 109 cells) |
Storage |
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Biotin-Antibody Mix |
100 μL |
200 μL |
4°C |
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Streptavidin Beads |
1 mL |
2 mL |
4°C |
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Shipping: Blue ice Shelf life: 2 years |
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