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Mitoxantrone HCl

Catalog No.
B2114
Topoisomerase II inhibitor, anti-neoplastic drug
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$61.00
In stock
Evaluation Sample
$30.00
In stock
50mg
$55.00
In stock
100mg
$94.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

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Email: [email protected]

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Background

Mitoxantrone HCl (CAS 70476-82-3) is an antineoplastic small molecule inhibitor targeting DNA topoisomerase II (Topo-II). Topo-II enzymes manage DNA topology during replication and transcription, facilitating chromosome segregation and relieving torsional stress. Mitoxantrone interferes with Topo-II-mediated DNA cleavage and ligation, inducing double-strand DNA breaks and chromatin rearrangement, thus disrupting DNA synthesis and cell cycle progression. It modulates activity of immune cells including T cells, B cells, and macrophages. Research demonstrates mitoxantrone's apoptotic or senescence-inducing effects in normal human cell models. Its uses in biomedical research include studies on leukemia, multiple sclerosis, and pancreatic cancer cell viability.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt517.4
Cas No.70476-82-3
FormulaC22H29ClN4O6·HCl
Solubilityinsoluble in EtOH; ≥51.53 mg/mL in DMSO; ≥2.97 mg/mL in H2O with ultrasonic
Chemical Name1,4-dihydroxy-5,8-bis[2-(2-hydroxyethylamino)ethylamino]anthracene-9,10-dione;dihydrochloride
SDFDownload SDF
Canonical SMILESC1=CC(=C2C(=C1NCCNCCO)C(=O)C3=C(C=CC(=C3C2=O)O)O)NCCNCCO.Cl.Cl
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

DPSCs and HDFs

Preparation method

The solubility of this compound in DMSO is > 18.2 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

5, 20, 50, 100 and 150 nM

Applications

Mitoxantrone HCl almost completely inhibited DPSCs and HDFs proliferation without causing significant decrease in cell viability after 6 days. Mitoxantrone HCl, at higher doses, i.e. 100 nM and 150 nM, significantly decreased DPSCs and HDFs viability after 3 days. In addition, Mitoxantrone HCl at doses over 50 nM significantly increased the activity of caspases 3/7 and the level of puma, inducing DPSCs and HDFs apoptosis.

Animal experiment [2]:

Animal models

Mice bearing PAC120 and HID xenografts

Dosage form

1 mg/kg; i.p.; once per 3 weeks

Applications

In mice bearing PAC120 xenografts, Mitoxantrone HCl was well tolerated, although it caused a weight loss of 10% or less. However, it did not inhibit tumor growth. In mice bearing HID xenografts, Mitoxantrone HCl transiently inhibited tumor growth with the optimal effect 3 weeks after the start of treatment. After 30 days, Mitoxantrone HCl no longer exhibited any inhibitory effect on tumor growth.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Seifrtova M, Havelek R, Soukup T, Filipova A, Mokry J, Rezacova M. Mitoxantrone ability to induce premature senescence in human dental pulp stem cells and human dermal fibroblasts. J Physiol Pharmacol. 2013 Apr;64(2):255-66.

[2]. Oudard S, Legrier ME, Boyé K, Bras-Gonalves R, De Pinieux G, De Cremoux P, Poupon MF. Activity of docetaxel with or without estramustine phosphate versus mitoxantrone in androgen dependent and independent human prostate cancer xenografts. J Urol. 2003 May;169(5):1729-34.

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