Mitochondrial Membrane Potential and Apoptosis Detection Kit

Annexin V is a Ca²⁺-dependent phospholipid-binding protein that binds specifically to phosphatidylserine (PS). PS is normally located medial to the lipid bilayer of the cell membrane, but in the early stages of apoptosis, PS can be flipped from the medial side of the cell membrane to the outer surface of the cell membrane, where it is exposed to the extracellular environment. Annexin V has a high affinity for PS, and the occurrence of apoptosis can be detected by fluorescein labeling with Annexin V.

The Mito-Tracker Red CMXRos, also known as MitoMark Red I, is an oxidized red fluorescent probe that specifically labels mitochondria. Mito-Tracker Red CMXRos can cross the cell membrane of living cells and aggregate specifically on the mitochondria of the cell. When the mitochondrial membrane potential decreases, the red fluorescence signal of the Mito-Tracker Red CMXRos also decreases, and the mitochondrial damage can be judged based on the strength of the fluorescent signal.

Mitochondrial Membrane Potential and Apoptosis Detection Kit is a kit for detecting apoptosis and mitochondrial membrane potential by the apoptosis green fluorescent probe Annexin V-FITC and the mitochondrial membrane potential red fluorescent probe Mito-Tracker Red CMXRos. Hoechst 33342 is also available for staining nuclei. After staining of this kit, the live cells are positive for red fluorescence and negative for green fluorescence; Apoptotic cells, on the other hand, are positive for green fluorescence and significantly weakened or negative for red fluorescence.

Figure 1: Diagram of the detection effect of this kit