MitoB

MitoB (CAS No.: 1247025-84-8) is a mitochondria-targeted ratiometric probe widely used in biomedical research to quantitatively assess hydrogen peroxide levels within the mitochondrial matrix of living systems. Functioning as an exomarker of mitochondrial reactive oxygen species, MitoB selectively accumulates in mitochondria driven by its lipophilic cationic moiety and reacts with hydrogen peroxide to form a phenolic product, enabling precise quantification of oxidative status through measurement of the product-to-parent ratio by mass spectrometry-based approaches. This mechanism provides a robust and sensitive means to monitor dynamic changes in redox signaling and oxidative stress in vivo, making it particularly valuable for studies of mitochondrial dysfunction, redox biology, and related pathologies. MitoB has been extensively applied in cell-based assays and animal models to investigate the role of hydrogen peroxide in signaling pathways and disease progression, including metabolic disorders and neurodegenerative conditions. While specific activity metrics such as IC50 or EC50 are not typically applicable to this type of analytical probe, its effective use generally falls within low micromolar ranges depending on experimental design. Overall, MitoB serves as a powerful tool for elucidating mitochondrial reactive oxygen species dynamics and supports drug discovery efforts targeting oxidative stress-associated mechanisms.