LY2584702 Tosylate

LY2584702 Tosylate (CAS No.: 1082949-68-5) is a synthetic small-molecule, ATP-competitive selective kinase pathway probe that primarily targets p70 ribosomal S6 kinase p70S6K, and is commonly used to dissect the functions and upstream/downstream networks of the PI3K–AKT–mTORC1–p70S6K–S6 axis in translational regulation, cell growth, and lipid metabolism; at the enzymatic level, it exhibits potent inhibition in the low nanomolar range with high kinase selectivity, and at the cellular level, it can reduce the phosphorylation signal of ribosomal protein S6 in the submicromolar to low micromolar range, as validated in models such as HCT116 colon-derived cells; in metabolic research settings related to mTORC1 hyperactivation, it can reduce triacylglycerol accumulation and apolipoprotein B secretion in TSC2-deficient or knockdown HepG2 hepatocyte models, and inhibits self-renewal at micromolar levels in primary mouse bone marrow stromal cell cultures, consistent with the blockade of p70S6K-driven anabolic programs; in in vivo studies, it is commonly used in mouse xenograft models to examine pathway dependence and target validation, with administration generally in the milligram-per-kilogram range and pharmacological indicators such as slowed tumor growth observed; typical experimental applications include biochemical enzyme activity and substrate phosphorylation readouts, cellular detection of phosphorylated S6 signaling, and small-molecule screening and mechanistic studies focused on p70S6K signal regulation, with the concentration or dose used in specific experiments typically depending on the research objective and experimental design; this product is positioned as a research tool compound for life science and early-stage drug discovery, strictly limited to laboratory research use and not for human or veterinary use.