L-Ascorbic Acid 2-phosphate (magnesium salt)

L-Ascorbic Acid 2-phosphate (magnesium salt) (CAS No.: 113170-55-1) is a chemically stabilized, phosphorylated derivative of vitamin C widely utilized in biochemical and cell-based research as a long-acting antioxidant and pro-ascorbate compound. As a member of the ascorbyl phosphate class, it resists rapid oxidative degradation and serves as a sustained intracellular source of ascorbate following enzymatic dephosphorylation, thereby supporting redox homeostasis and modulating oxidative stress–responsive pathways. This compound has been shown to promote collagen synthesis and extracellular matrix formation through regulation of gene expression associated with collagen production, and to enhance osteogenic differentiation in mesenchymal-derived cells, including human adipose stem cells, as evidenced by increased alkaline phosphatase activity and upregulation of transcription factors such as RUNX2. In addition, it has been reported to stimulate hepatocyte growth factor production and to function as a free radical scavenger, implicating roles in pathways related to cellular proliferation, differentiation, and antioxidant defense. In vitro, its biological activity is typically observed across low micromolar to millimolar concentration ranges depending on the experimental system, with dosing optimized according to specific assay conditions. L-Ascorbic Acid 2-phosphate (magnesium salt) is therefore frequently employed as a supplement in cell culture media, particularly in studies of stem cell differentiation, tissue engineering, and oxidative stress biology, as well as in compound screening platforms investigating redox-regulated signaling and matrix-related cellular processes.