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Kifunensine

Catalog No.
B7315
Inhibitor of class I α-mannosidases that inhibits glycoprotein processing
Grouped product items
SizePriceStock Qty
1mg
$95.00
Ship with 5-10 days
5mg
$349.00
Ship with 5-10 days
10mg
$507.00
Ship with 5-10 days
50mg
$1,901.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Kifunensine (CAS 109944-15-2) is a selective inhibitor of class I α-mannosidases, enzymes critical for glycoprotein processing and folding. It primarily inhibits human endoplasmic reticulum α-1,2-mannosidase I and Golgi α-mannosidases IA, IB, and IC with Ki values of approximately 130 nM and 23 nM, respectively. Additionally, it suppresses plant-derived α-1,2-mannosidase I at IC50 values between 20–50 nM. By blocking α-mannosidase I activity in the endoplasmic reticulum (ER), kifunensine prevents the ER-associated degradation of misfolded glycoproteins, thus serving as an investigative tool in studies of protein quality control pathways and glycoprotein biosynthesis.

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt232.19
Cas No.109944-15-2
FormulaC8H12N2O6
SolubilitySoluble in H2O
Chemical Name(5R,6R,7S,8R,8aS)-6,7,8-trihydroxy-5-(hydroxymethyl)hexahydroimidazo[1,2-a]pyridine-2,3-dione
SDFDownload SDF
Canonical SMILESVO[C@@H]([C@H]1O)[C@@H](NC2=O)N(C2=O)[C@H](CO)[C@H]1O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

Hybridoma cells expressing a human IgG1 monoclonal antibody against a tumor vascular associated antigen

Reaction Conditions

2 μg/mL kifunensine for 4 d incubation

Applications

Kifunensine treatment significantly reduced the lentil lectin binding. Furthermore, kifunensine was the most effective among the glycosylation inhibitors tested in producing antibodies containing oligomannose residues without fucose.

Animal experiment:[1]

Animal models

BALB/c mice

Dosage form

5 mg/kg

Injected via the tail vein

Applications

The serum levels of antibody in mice were not significantly altered up to 168 h following injection. The use of kifunensine provided a simple and rapid method for the production of antibodies with increased antibody-dependent cell mediated cytotoxicity (ADCC) without the time-consuming need to re-engineer either the antibody molecule or the host cell line.

Note

The technical data provided above is for reference only.

References:

1. Zhou Q, Shankara S, Roy A, et al. Development of a simple and rapid method for producing non-fucosylated oligomannose containing antibodies with increased effector function. Biotechnology and Bioengineering, 2008, 99(3): 652-665.

Quality Control

Quality Control & MSDS

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Chemical structure

Kifunensine