INH14

INH14 (CAS No.: 200134-22-1) is a small-molecule, cell-permeable inhibitor of IκB kinase α and β (IKKα/IKKβ) that modulates innate immune signaling pathways, particularly those associated with Toll-like receptor (TLR)–mediated inflammatory responses. It exhibits inhibitory activity against IKKα and IKKβ at low micromolar levels, with reported IC50 values of 8.97 μM and 3.59 μM, respectively, and suppresses IKK-dependent downstream signaling cascades, including the NF-κB pathway. INH14 also acts downstream of the TAK1/TAB1 complex, influencing TLR2-associated signaling events and altering the expression of pathway-related proteins. In cellular systems, it has been shown to reduce NF-κB activity and impair processes such as cell migration, while in murine models it attenuates inflammatory responses, including decreased TNF-α production following lipoprotein-induced stimulation. These properties position INH14 as a useful research tool for investigating kinase-mediated regulation of inflammation, signal transduction in innate immunity, and pathway interactions relevant to cancer and inflammatory biology. Experimental applications typically involve concentration ranges and treatment conditions optimized according to specific assay systems and study objectives.