Immunostaining Permeabilization Buffer with Saponin

Immunostaining Permeabilization Buffer (Saponin) is suitable for various in situ detection applications, including immunostaining of cell samples, frozen sections, and paraffin-embedded sections. Its function is to expose target sites such as antigens and nucleic acids, allowing antibodies, probes, or labeling reagents to enter cells more easily, thereby ensuring effective staining and detection. This product is a ready-to-use working solution and requires no dilution.

This product has strong permeabilization capability and is recommended for permeabilizing cells in various routine applications such as immunofluorescence, immunohistochemistry, immunocytochemistry, and flow cytometry. It is particularly suitable for detecting cell membrane proteins. It does not dissolve the cell membrane nor affect light scattering in flow cytometry. However, it is not recommended for detecting proteins inside the nucleus or mitochondria.

Saponin is a specific detergent that dissolves cholesterol in the cell membrane, enabling selective permeabilization of the cell membrane. Its advantage lies in its suitability for detecting cell membrane proteins, especially for detecting cell surface marker proteins by flow cytometry. However, permeabilization may be less effective in cells with low cholesterol content, and it cannot permeabilize the nuclear membrane or mitochondrial membrane, which have very low cholesterol levels. For lectin detection, permeabilization buffers containing non-specific detergents such as Triton X-100 yield better results than buffers containing mainly Saponin.

Immunostaining results for target cell membrane proteins in samples treated with this Immunostaining Permeabilization Buffer (Saponin) are generally comparable to or significantly enhanced relative to conventional permeabilization buffers.