Immnol Staining Wash Buffer (10X)

Immunostaining Wash Buffer (10X) can be used for washing after fixation, primary antibody incubation, or secondary antibody incubation in immunostaining techniques such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (IC). Proper washing reduces background and enhances the signal-to-noise ratio.

This immunostaining wash buffer contains an appropriate amount of Triton X-100, which permeabilizes cell membranes. Cells or tissue samples treated with this wash buffer enable the detection of intracellular target proteins, including those in the cytoplasm and nucleus.

Based on a wash volume of 0.1–1 mL of Immunostaining Wash Buffer (1X) per sample, one 100 mL package of this product can be used for 1,000–10,000 sample washes.