HyperTrap SP HP Column
HyperChrom SP HP Agarose is a strong cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions.HyperChrom SP HP Agarose has excellent scale-up performance: 1) Highly crosslinked agarose base with excellent rigidity, resulting in high process flow rates and improved process efficiency at low backpressure. 2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. 3) Fine particle size design to improve resolution.
HyperTrap SP HP Column is a preloaded column with HyperChrom SP HP Agarose as the chromatography mediumIt is used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc. The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap SP HP Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap SP HP Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Strong cation exchange |
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Ligation |
- (CH2)3SO3- (sulfopropyl). |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
34 μm |
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Ion load |
0.15-0.20 mmol H+ /mL chromatography medium |
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Dynamic load |
>50 mg β-lactoglobulin/mL chromatography medium* |
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Flow rates are recommended |
90-150 cm/h |
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Maximum flow rate |
200 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability ** |
2-14 |
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Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
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Intolerant to solvents |
Oxidant, cationic detergent |
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*Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 150 cm/h, test buffer: 0.1 M Citrate solution, pH 8.0, test sample: 4 mg/m Lβ-lactoglobulin sample, when the penetration of β-lactoglobulin reaches 10%,β-lactoglobulin loading volume per unit media volume (mL) (mg). **After 7 days of storage of chromatography medium at 40°C and pH 2-14, its physicochemical properties and functions did not change significantly. ***30% and 75% are v/v, volume ratio. |
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