HyperTrap SP FF Column
HyperChrom SP FF Agarose is a strong cation exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. HyperChrom SP FF Agarose has excellent scale-up performance: 1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low backpressure. 2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperTrap SP FF Column is a preassembled column with HyperChrom SP FF Agarose as the chromatography medium, which is used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc. The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap SP FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap SP FF Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Strong cation exchange |
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Ligation |
- (CH2)3SO3- (sulfopropyl). |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
90 μm |
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Ion load |
0.18-0.25 mmoll H+/mL chromatography medium |
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Dynamic load |
>50 mg β-lactoglobulin/mL chromatography medium* |
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Flow rates are recommended |
300-600 cm/h |
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Maximum flow rate |
700 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability ** |
2-14 |
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Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
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Intolerant to solvents |
Oxidant, cation detergent |
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* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M citrate solution, pH 3.0; test sample: 4 mg/ml β-lactoglobulin sample, when the penetration of β-lactoglobulin reaches 10%, the amount of β-lactoglobulin per unit volume of media (mL) (mg). **After 7 days in the environment of chromatography at 40°C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
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