HyperTrap Protein G 4FF Column
HyperChrom Protein G 4FF Agarose is an affinity chromatography medium made by coupling Protein G to a substrate by CNBr activation. Protein G can bind to the Fc region of IgG of a variety of mammals and also has a weak affinity for its Fab region. Compared to Protein A, Protein G has a broader binding spectrum. Therefore, HyperChrom Protein G 4FF Agarose can be used to isolate and purify immunoglobulins and their fragments from different liquid samples such as cell culture media or serum. HyperChrom Protein G 4FF Agarose can also be used to isolate immune complexes.
HyperTrap Protein G 4FF Column is a ready-to-use chromatography column with HyperChrom Protein G 4FF Agarose as the packing material, which has the advantages of high flow rate, high load capacity, easy regeneration, low cost and easy amplification.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap Protein G 4FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap Protein G 4FF Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Affinity chromatography media |
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Ligation |
Protein G (E. coli recombinant expression). |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
90 μm |
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Ligand density |
~2 mg Protein G/mL chromatography medium |
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Dynamic load |
≥ 20 mg human IgG/mL chromatography medium* |
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Flow rates are recommended |
90-300 cm/h |
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Maximum flow rate |
400 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability ** |
3-10 |
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Chemical resistance |
Common aqueous solution, 30% isopropanol, 75% ethanol, 6 M guanidine hydrochloride, 8 M urea |
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*Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 200 cm/h, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: 2 mg/m IgG sample, when the penetration of IgG reaches 10%, unit medium volume (mL) IgG loading volume (mg). **After 7 days of storage in the environment of 40 °C and pH 3-9, the physicochemical properties and functions of the chromatography medium did not change significantly. *** 30% and 75% are the volume ratio (v/v). |
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