HyperTrap Phenyl 6FF (HS) Column
HyperChrom Phenyl 6FF (HS) Agarose is a class of hydrophobic interaction chromatography media that takes advantage of differences in the hydrophobic properties and amount of different molecules under specific conditions. The HyperChrom Phenyl 6FF (HS) Agarose series has excellent scale-up performance: 1) Highly crosslinked agarose base frame with excellent rigidity, resulting in high process flow rates and improved process efficiency at low back pressure. 2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. 3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperTrap Phenyl 6FF (HS) Column is a preloaded column with HyperChrom Phenyl 6FF (HS) Agarose as the chromatography medium, which is mainly used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap CM HP Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap Phenyl 6FF (HS) Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Hydrophobic interactions |
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Ligation |
R-O-CH2-CH(OH)-CH2-O-C6H5 |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
90 μm |
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Ligand density |
45 μmol phenyl group/mL chromatography medium (HS) |
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Flow rates are recommended |
150-300 cm/h |
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Maximum flow rate |
600 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability* |
2-14 |
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Solvent resistant |
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 10% ethylene glycol**, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
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Intolerant to solvents |
Oxidant |
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* After 7 days of storage of chromatography medium at 40°C and pH 2-14 environment, its physicochemical properties and functions did not change significantly. **30%, 75% and 10% are v/v, volume ratio. |
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