HyperTrap Geldex 200 PG Column
Geldex PG is a series of gel filtration chromatography media for cross-linked agarose scaffolds that take advantage of the molecular weight differences of different molecules to separate them. The series consists of three chromatography media for different separation ranges. This series of chromatography media can be well used for the separation and purification of a variety of biomolecules, such as: peptides, polysaccharides, recombinant proteins, nucleic acids, viruses, etc.
The Geldex PG series of chromatography media offers excellent scale-up throughput: (1) The improved Geldex base frame is more rigid, so it can achieve higher process flow rates and improve process efficiency at lower backpressures. (2) The modified Geldex substrate has better chemical resistance and is compatible with a wide range of buffer environments. (3) Fine particle size design to improve resolution.
Both the Geldex PG series are available in two prepacked column sizes, with 16 mm and 26 mm I.D. and 600 mm bed height, packed with 16/700 and 26/700 empty columns, respectively. Taking globulin as a reference, the separation range of this product is 10-600 kD, which has the characteristics of low non-specific adsorption and good chemical stability.
1. HyperTrap Geldex 200 PG Column prepacked column parameters
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Column volume |
120 mL |
320 mL |
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Bed dimensions |
16 x 600 mm |
26 x 600 mm |
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Sample volume* |
≤5 mL |
≤13 mL |
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Pressure-resistant |
0.5 MPa |
0.3 MPa |
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Flow rate** |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30℃ |
4 - 30℃ |
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Storage conditions |
Store the components at 4℃ for 5 years. |
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*It can be adjusted according to the actual situation **This flow rate is the recommended common flow rate, the pressure resistance and flow rate of different types of chromatography resins in the prepacked column will be different, please refer to the product manual of the corresponding chromatography resin. |
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2. HyperTrap Geldex 200 PG Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Gel filtration |
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Scaffolding |
High degree of cross-linking agarose with dextran linker arm |
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Separation range |
10-600 kD |
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Main particle size distribution* |
22-44 μm |
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Flow rate is recommended |
10-50 cm/h |
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Maximum flow rate |
100 cm/h |
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Pressure-resistant |
0.3 MPa |
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Operating temperature |
4 - 30℃ |
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pH stability** |
2-14 |
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Chemical resistance |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
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*Percentage of distribution in range≥80%. **After 7 days of storage at 40°C, pH 2-14, the physicochemical properties and functions of the chromatography medium showed no significant change ***v/v, volume ratio. |
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