HyperTrap CM FF Column
HyperChrom CM FF Agarose is a weak cation exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom CM FF Agarose chromatography media exhibits an unexpected separation and purification effect. HyperChrom CM FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperTrap CM FF Column is a preassembled column with HyperChrom CM FF Agarose as the chromatography medium, which is used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap CM FF Column preloaded column parameters
|
Pre-assembled column specification/name |
1 mL |
5 mL |
|
Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
|
Withstand pressure |
0.3 MPa |
0.3 MPa |
|
Workflow Speed* |
1 mL/min |
1-3 mL/min |
|
Operating temperature |
4 - 30°C |
4 - 30°C |
|
Storage condition |
Store the components at 4℃ for 5 years. |
|
|
*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
||
2. HyperTrap CM FF Column chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Weak cation exchange |
|
Ligation |
-O-CH2COO- |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ion load |
0.09-0.13 mmol H+ /mL chromatography medium |
|
Dynamic load |
> 50 mg lysozyme/mL chromatography medium* |
|
Flow rates are recommended |
300-600 cm/h |
|
Maximum flow rate |
700 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, cationic detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M citrate solution, pH 3.0; test sample: 4 mg/mL lysozyme sample, when the penetration of lysozyme reaches 10%, the lysozyme sample volume per unit volume (mL) (mg). **After 7 days in the environment of chromatography at 40°C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
|