HyperTrap Butyl 4FF Column
HyperChrom Butyl 4FF Agarose is a hydrophobic interaction chromatography medium that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. HyperChrom Butyl 4FF Agarose has excellent scale-up performance:(1) a highly crosslinked agarose base frame with excellent rigidity, resulting in high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperTrap Butyl 4FF Column is a pre-assembled column with HyperChrom Butyl 4FF Agarose as the chromatography medium, which is mainly used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap Butyl 4FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap Butyl 4FF Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Hydrophobic interactions |
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Ligation |
R-O-CH2-CH(OH)-CH2-O-(CH2)3-CH3 |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
90 μm |
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Ligand density |
40 μmol Butyl group/mL chromatography medium |
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Flow rates are recommended |
150-250 cm/h |
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Maximum flow rate |
400 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability* |
2-14 |
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Solvent resistant |
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 1 M NaOH, 6 M guanidine hydrochloride |
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Intolerant to solvents |
oxidant |
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* After 7 days of storage of chromatography medium at 40oC and pH 2-14 environment, its physicochemical properties and functions did not change significantly. **30% and 75% are v/v, volume ratio. |
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