HyperScript™ III RT SuperMix for qPCR

HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity. In addition, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA. HyperScript™ III Reverse Transcriptase has an enhanced affinity for templates and is suitable for reverse transcription of small amounts of template as well as low-copy genes, you can use PCR to amplify cDNA up to 12.3 kb.

The HyperScript™ III RT SuperMix for qPCR is designed for two-step qRT-PCR assays, 5 × HyperScript™ III SuperMix contains all components for reverse transcription except template RNA. The maximum volume of the RNA template can be up to 80% of the total volume, making the kit ideal for reverse transcription of low concentrations RNA templates. The 5X HyperScript™ III SuperMix doesn’t freeze at -20°C and is easy to use.

This product has been optimized for qPCR, especially the ratio of Oligo (dT)₂₃VN primers /Random primers, the cDNA synthesis can be initiated from all regions of the RNA transcript with the same efficiency, ensuring maximum authenticity and reproducibility of qPCR results. The reverse transcription products can be employed in SYBR Green or probe-based qPCR, you can choose appropriate reagents for gene expression analysis according to your purpose.