Hyper Gel Red

Hyper Gel Red is a sensitive and stable dye for double-stranded DNA (dsDNA), single-stranded DNA (ssDNA) and RNA. It features high stability (higher than that of SYBR Green series dyes), low mutagenicity, no significant toxicity, and environmental safety. The agarose gel after use can be directly discarded without special treatment. It is suitable for both agarose gels and polyacrylamide gels.

Hyper Gel Red is a substitute for EB, with basically the same usage conditions as EB. It is excited by ultraviolet light (wavelength around 300 nm) and emits red fluorescence. The mutagenicity of Hyper Gel Red is much lower than that of EB. EB exhibits strong mutagenicity in various mutagenicity tests and is highly harmful to the human body. In contrast, Hyper Gel Red shows weak mutagenicity only at a concentration of 20 μg/mL, which is much higher than the working concentration.

Moreover, due to its special molecular structure, Hyper Gel Red cannot pass through the living cell membrane and enter the cell. Hyper Gel Red also has high sensitivity for samples with low concentrations. For small-molecular-weight nucleic acids, Hyper Gel Red has higher detection sensitivity compared with EB and SYBR Safe.

Hyper Gel Red can be used in multiple ways: it can be added either before or after the heating of the agarose gel, or post-staining method can be adopted. We provide a 10000X concentrated solution. You can dilute it at a ratio of 1:10000 into 1X TBE or 1X TAE, add agarose powder and heat to dissolve; alternatively, dilute it at a ratio of 1:10000 into the dissolved agarose gel before it solidifies. You can also choose to incubate the gel in Hyper Gel Red staining solution (diluted at a ratio of 1:3333 with 1X TBE or 1X TAE) after electrophoresis. When using polyacrylamide gel electrophoresis, please adopt the post-staining method, and the staining time is about 30 minutes to 1 hour.

Hyper Gel Red bound to DNA or RNA can be effectively removed by using a gel extraction kit or phenol-chloroform extraction, without affecting subsequent experiments.

Hyper Gel Red has low mutagenicity and no obvious toxicity, making it a suitable substitute for EB.

Hyper Gel Red has higher detection sensitivity for small-molecular-weight nucleic acids.

The usage methods and conditions of Hyper Gel Red are basically the same as those of EB, without the need to replace instruments.