HyperChrom Resilient ARPA Agarose
HyperChrom Resilient ARPA Agarose is an affinity chromatography medium for antibody purification that is separated by a specific interaction between antigen and antibody. This chromatography medium is used to process high concentrations of monoclonal antibodies.
HyperChrom Resilient ARPA Agarose has the characteristics of high load, high flow rate and alkali resistance: (1) High flow rate and high dynamic binding load shorten the processing time. (2) The modified alkali-resistant rProtein A ligand can withstand 0.5M NaOH for CIP.
HyperChrom Resilient ARPA Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
Alkali resistant Protein A |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
85 μm |
|
Dynamic load |
≥ 60 mg human IgG/mL chromatography medium* |
|
Flow rates are recommended |
90-500 cm/h |
|
Maximum flow rate |
700 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
2-40℃ |
|
pH stability ** |
2-14 |
|
Chemical resistance |
A water-soluble buffer commonly used in Protein A chromatography |
|
*Dynamic load measurement conditions: column loading height: 10 cm, retention time 6 min, test buffer: 0.02 M NaH2PO4 solution, 0.15 M NaCl, pH 7.4, lgG loading volume per unit media volume (mL) (mg) when lgG penetration reaches 10%. **After 7 days of storage of chromatography medium at 40 °C and pH 2-14, its physicochemical properties and functions did not change significantly. |
|
| Components | PC2004-25 mL | PC2004-100 mL |
| HyperChrom Resilient ARPA Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||